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The pellets were washed twice and supplement with fresh cellobiose to initiate another cycle.
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Cells were supplemented with fresh medium after 5 h.
Cells were supplemented with fresh improved medium every 3 4 days, depending on the requirements, until confluence was achieved.
The treatments were three diets containing guinea grass (Panicum maximum) and cassava chips supplemented with fresh cassava foliage (FCFd), wilted cassava foliage (WCFd) or cassava hay (CHd).
Although my Ethiopian diet consists of these dishes only on very special occasions, my nutrient intake increases significantly when training at altitude with meat, fish and eggs supplemented with fresh fruit, vegetables and grains to fuel my many miles.
To achieve this, beyond passage 5, cells were supplemented with fresh media every 3 days and passaged at 90% confluence (typically once per week as proliferation rate slowed) without dilution (passage ratio 1 1).
Fig. 1 The pictorial presentation of test pathogen a Culture of P. acne, an anaerobic bacteria, growth maintained on anaerobioc blood agar supplemented with fresh sheep blood.
Each well was supplemented with fresh hESCM every second day.
Cultures were supplemented with fresh medium plus RAPA as needed.
The dishes were then washed twice with PBS and supplemented with fresh DMEM.
The cells were supplemented with fresh macrophage medium on Day 3 after culture initiation.
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