In order to visualize iodine formation 10 μl 4% (w/v), starch solution was supplemented to the test solution giving a dark-blue color.
For this purpose, known quantities of brimonidine tartrate (0.1, 10, 50 μg/mL) were supplemented to the sample solution previously analyzed.
In the standard addition method known quantities (50, 100 and 150%) of moxifloxacin and prednisolone were supplemented to the sample solution previously analysed and then experimental and true values were compared.
For this purpose, known quantities of pheniramine maleate (150, 600, 1200 μg mL-1) and naphazoline hydrochloride (12.5, 50, 100 μg mL-1) were supplemented to the sample solution previously analyzed.
The minimal medium was supplemented to the feed solution to a final hexose (glucose, mannose and galactose) concentration of 50 g L-1 and the salts, vitamins and trace elements were correspondingly scaled up.
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The nutrient solution was supplemented to the pots every other day, whereas the ALA was supplemented every day.
Amino acids (Sigma-Aldrich, Finland) were supplemented to the worts from sterile-filtered stock solutions prepared in deionized water.
Interestingly, the inhibition of YtA root growth was observed to be rescued when 100 nM Vitamin C was supplemented in the solution (Fig. 7C, D).
The initial section of the scale-up study involved the selection of non-penetrating additives to supplement VSbasic and increase the solution's total solute concentration.
Crystals were cryoprotected by transfer to the reservoir solution supplemented with 30% ethylene glycol, and flash cooled in liquid nitrogen.
