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A549 human lung epithelial cells were used and under the release of an epithelial growth supplement mix, there was a significant improvement on the epithelial proliferation (p < 0.01).
and HAT supplement mix (Sigma).
Osteogenic and chondrogenic differentiation capabilities of STRO-1+ cells from the antler growth region were tested by incubating them immediately after sorting for 3 4 weeks in osteoblast growth medium (PromoCell) + supplement mix (C-27001, PromoCell) and in osteoblast and chondroblast differentiation media from the hMSC functional identification kit (SC006, R&D Systems).
Primary human myoblast cultures were isolated by protease digestion from fresh muscle biopsies (collagenase II, dispase1, trypsin/EDTA) and expanded in skeletal muscle growth medium including supplement mix (PromoCell, Heidelberg, Germany), 10% FCS, glutamine (3 mM) and gentamycin (40 µg/ml).
The cells were cultured in T25 flasks at a density of 1×106 cells per ml in ECGM medium supplemented with 10% v/v FCS and Supplement Mix containing 100 IU/ml polymyxin B in an incubator at 37°C in a humidified atmosphere with 5% CO2.
To investigate the influence of different culture media on the proliferation of STRO-1+ cells, we cultivated cells for one month in Dulbecco's minimal eagle medium [DMEM (Gibco) + 10% FCS], osteoblast growth medium (OB) + supplement mix (both Promocell) or NeuroBasal medium (NB/Gibco) containing 50 ng/ml nerve growth factor (NGF 7S/Invitrogen).
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Detailed advice on dose incrementation and supplement mixing with food or fluid will be provided by the trial dietician (KL).
Transfection medium was removed after incubation for 6 h and substituted by ECGM supplemented with ECGM-supplement mix and 1% penicillin/streptomycin/amphotericin B (Sigma-Aldrich).
Hippocampal neurons were transfected on DIV3 (for morphometry) or DIV5-8 (for videomicroscopy) as follows: for each coverslip, plasmid DNA (2 μg) and Lipofectamine 2000 (1.25 μl, Life Technologies) in Neurobasal medium were combined and incubated for 30 min. After the addition of complete Neurobasal medium containing B27 supplement, the mix was applied onto the neuronal culture for 3 hr at 37°C.
Most standard vitamin or mineral supplement companies mix their products with lots of artificial colors, preservatives and allergens in order to preserve them.
A two-week supply of tablets were provided at enrolment, and the mothers instructed on administering tablets to their child by crushing it and mixing it with food, and assuring that the child ate all the food the supplement was mixed in.
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CEO of Professional Science Editing for Scientists @ prosciediting.com