They are often the creations of multiple sets of national primary (enabling) and secondary (detailed implementing) legislation supplemented as necessary by further provisions at ministerial, inter-ministerial, regional, provincial and local levels of government.
Briefly, the rats were anesthetized by intraperitoneal injection of ketamine hydrochloride (100 mg/kg) and xylazine (5 mg/kg) supplemented as necessary.
Strains were cultured in nitrogen base liquid (NBL) or on nitrogen base agar (NBA) minimal media supplemented as necessary with the requisite amino acids and bases (100 µg/ml) [51], [52].
Rats were anesthetized with a ketamine-xylazine (4:1) cocktail (50 mg/kg ip) and supplemented as necessary for reflexive response.
Growth was in YPD or in synthetic defined (SD) medium with 2% (w/v) galactose, supplemented as necessary [ 59].
To identify and summarise relevant research evidence using existing synthesised evidence sources (systematic reviews) supplemented as necessary by a rapid systematic review of primary research.
Growth occurred in yeast potato dextrose or in synthetic defined (SD) medium with 2% (w/v) glucose or galactose, supplemented as necessary [ 57].
Six animals were anaesthetized with a mixture of ketamine and xylazine (73.3 and 7.3 mg/kg intraperitoneal, respectively, supplemented as necessary) and placed in a stereotaxic frame.
Ten adult male Wistar rats (240 320 g; Harlan, Loughborough, UK) were anaesthetised with ketamine and xylazine (73.3 and 7.3 mg/kg i.p., respectively, supplemented as necessary) and placed in a stereotaxic frame.
Anemia management will be carried out according to a standardized nursing-led anemia protocol aiming for a goal hemoglobin of 110 – 120 g/L utilizing erythropoietic stimulating proteins and iron supplements as necessary [ 21].
