However, even if penetration of ingredients into the tooth surface or ion leakage into deeper zones of the superficial strata of the dentin occurred [3], this was not sufficient to affect the adhesion of the final restoration, different from previous results [2, 19].
Individual raphe populations differ in their radial position (stratification) within the paramedian basal plate, occupying positions at either the periventricular, intermediate or superficial strata.
The raphe populations normally migrate radially into the local mantle, and differentiate there, occupying, therefore, a paramedian position adjacent to the glial raphe, either periventricularly or within the intermediate or superficial strata.
This cluster is characterized by a stereotyped distribution of its elements in the periventricular, intermediate and superficial strata, largely within the four rostralmost rhombomeres (Is, r1 r3), to which must be added the mentioned minor population in r4 and the small subpopulation found within the periaqueductal gray of the caudal midbrain (Figs. 1, 2a f, 3).
These pontomedullary and medullary periventricular populations decrease in importance, or disappear, at subsequent stages, either due to downregulation of the markers or depletion of the stratum via radial migration into the intermediate and superficial strata (Figs. 10a, c, e, g, 11a, c, e, g, 12a, c, e, g).
The superficial strata retained morphologically normal characteristics.
In each subject, a series of images was collected at increasing depth, from superficial stratum corneum to papillary dermis.
Unlike EP1, however, little EP2 was observed in the superficial stratum spinosum.
A smaller number (15%) innervate the most superficial stratum opticum (SO).
At E12.5 Gand2 and Gata3 are generally weakly expressed in the intermediate stratum across the whole caudal cluster (Fig. 10a d), whereas there is stronger expression at the superficial stratum, particularly in its ventrolateral (parapyramidal) part.
Intact mucosa consists of three layers of epithelia, the more darkly stained basal layer, the middle layer, with larger and more compact cells, and the superficial stratum, with less compact cells and surrounded by mucus.
