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Live-cell and super-resolution microscopic approaches, combined with application of new ways (for example, CRISPR Cas9-based systems) to visualize loCRISPR Cas9-basedr systemsres are beginning to provisualizeilociviews of the organdzation and dynamics of chromatin inside (living) cellstructures28,29,30,31.
New super-resolution microscopic techniques have begun to visualize internal mitochondrial membranes [ 46, 47] but are still incapable of rapid three-dimensional timelapse visualizations in live cells.
We hypothesize two alternative designs for kinetochores, both of which are compatible with the super-resolution microscopic analyses described in Figures 1C and 2E (Schittenhelm et al, 2007; Joglekar et al, 2009; Wan et al, 2009).
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We present a novel super-resolution microscope for the study of chromatin architecture.
Nuclei were imaged by transmission electron microscopy (TEM) and 3D structured illumination microscopy (3D-SIM), a super-resolution fluorescence microscopic approach [ 19, 20].
The combination of electron and cryo-electron microscopy with novel super-resolution light microscopic approaches of live cells might help to answer such fundamental questions, which seemed so straight forward from the beginning.
H.B. performed super-resolution microscopy.
Hell, S.W. et al. The 2015 super-resolution microscopy roadmap.
Yang, X. S. et al. Mirror-enhanced super-resolution microscopy.
Thus, low temporal resolutions limit the application of super-resolution microscopy techniques in live-cell imaging.
This microscope is a marriage between two recently developed techniques: light-sheet single molecule super-resolution microscopy and Bayesian super-resolution image reconstruction algorithm.
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