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Chen, C. et al. Imaging and intracellular tracking of cancer-derived exosomes using single-molecule localization-based super-resolution microscope.
A super-resolution microscope can achieve a resolution of 250 nanometers; a single red blood cell, for a comparison, is about 8,000 nanometers wide.
Her research, possible only through the use of a super-resolution microscope, has implications for diagnosing and preventing birth defects and genetic defects.
With DNA-PAINT super-resolution microscopy and qPAINT analysis, researchers will be able to quantify the number of molecules at specific locations in the cell without the need to spatially resolve them, without requiring an expensive large super-resolution microscope.
In a tiny room in the sub-basement of MIT's Building 66 sits a customized, super-resolution microscope that makes it possible to see nanoscale features of a red blood cell.
STED measurements were performed on a modified commercial easy3D STED microscope (2C QUAD Scan easy3D Super-Resolution microscope, Abberior Instruments) with avalanche photodiodes (Single Photon Counting Module, SPCM-AQRH-13-FC, Excelitas Technologies) for detection.
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Super-resolution microscopes also exist such that single molecules within a sample can be imaged.
Because localization microscopes are super-resolution microscopes, the first 'proof' is generally to evaluate the optical resolution, defined as the shortest distance at which two point emitters can be distinguished (the Rayleigh Criterion).
The advent of super-resolution microscopes (like the structured illumination microscope) with enhanced resolving capacity allows researchers to combine the multiprotein localization capabilities of immunofluorescence with fine structural analysis in three dimensions.
Super resolution microscopy (Nikon Super resolution microscope, N-SIM) was used to study the endocytosis of NDs by cells at 37 and 4 °C.
"The Elyra PS.1 Super Resolution microscope will allow me to track and visualize cargo on a freight car moving along cellular rail roads from destination A to B in real time.
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