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Rieger, B. & Stallinga, S. The lateral and axial localization uncertainty in super-resolution light microscopy.
A new technique pushes the boundaries of super-resolution light microscopy.
He suspected that powerful new super-resolution light microscopy could reveal the unknown structure of biofilms.
Maglione, M. & Sigrist, S. J. Seeing the forest tree by tree: super-resolution light microscopy meets the neurosciences.
To this end, we subjected the brains of ok107/atg9 KD to transmission emission electron microscopy (EM) and super-resolution light microscopy (STED).
We combined fluorescent molecular tension sensors with super-resolution light microscopy to visualize traction forces within FAs with <100 nm spatial resolution.
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Utilizing a combination of super-resolution, light, and electron microscopy, we revealed that nanotubes are composed of chains of membranous segments harboring a continuous lumen.
Our successful imaging of gold particles suggests that guided STED could also be applied in correlative super-resolution light-electron microscopy, as gold beads are often used for image registration (reviewed in71,72).
The combination of electron and cryo-electron microscopy with novel super-resolution light microscopic approaches of live cells might help to answer such fundamental questions, which seemed so straight forward from the beginning.
We have witnessed light microscopy breaking the diffraction barrier, and in the future the spatial resolution will only improve, with the development of super-resolution light microscopes.
(3) Labeling would ideally permit visualization by high-resolution light microscopy approaches (such as confocal, two-photon, or super-resolution microscopy).
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