Sentence examples for super shift from inspiring English sources

Preincubation of the nuclear extract with p65 NF-κB antibody resulted in the super shift of the band.

To categorically demonstrate that the factor binding to Sp1 motif, present within the resistin gene, is indeed Sp1, super shift assays were carried out.

For super shift, proteins were incubated with anti-HIF-1α and anti-p65 mAb for 1h at 4°C prior to the binding assay.

The addition of anti-HIF-1α mAb resulted in an obvious super shift indicative of the union of HIF-1α to the DNA consensus region, suggesting the high DNA binding activity of HIF-1α transcription factor in TAT.

Super shift assays were done with HNF4α specific antibody sc-6556xx), HNF1α specific antibody sc-6547xx), aNFATFAT specific antibody sc-1149xx), all were purchased from Santa Cruz Biotechnology, Heidelberg, Germany and once again details are given in [31].

p21-5'-DBS was radio-labeled and was mixed with recombinant p53 and DNA competitor for EMSA analysis either in the presence or absence of PAb421 antibodies (for super shift) and/or purified recombinant NTD125.

Potential AP-1 components were identified by Western blot (Fig. 3C) and electrophoretic mobility-super shift assay (Fig. 3D).

The identity of the YY1-shifted species was confirmed by super-shift with an antibody against YY1.

Note: An unexpected but interesting scenario "super-shift" is consistently observed in our trials with V. cholerae FadR.

The bioinformatics analysis indicated two NF-κB binding sites on the Orm1 promoter, and a super-shift assay verified that NF-κB could bind the Orm1 promoter.

The fact that the FadR_vc protein gives consistently the super-shift bands for both fabA and fabB probes in the gel shift assays, is mostly attributed to the essence of its easy-forming the protein multimer (Fig. 3).