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Analysis of the tertiary structures showed that three essential domains of the enzyme including super secondary structure 8, the barrel region, and the large cleft remained native in Amyl-S1 and Amyl-S2.
The selected peptide sequences are leucine zipper motives from transcription factors and are known to bind to each other specifically by formation of a super secondary structure called coiled-coil.
The secondary and super secondary structures of MAP1138c protein were identified and analyzed using PROMOTIF.
The 69 sites were scattered over the 8 SSSUs (Super Secondary Structure Units) of the Oxidored_FMN domain, except for four sites (16, 20, 22 and 24) at the N-terminus.
For example, 5 critical amino acid sites were predicted for the subfamilyI/VI pair and distributed in SSSU (Super Secondary Structure Unit) 1, 3, 4 and 6, while 16 critical amino acids sites were predicted for the subfamilyI/VII pair and distributed in SSSU 1, 2, 3, 4, 5 and 7 (Additional file 7).
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The coiled-coil motif is another model system for understanding of molecular mechanisms that govern the super-secondary structure formation.
The database is particularly useful to quickly retrieve amino acid sequences that are compatible to any super-secondary structure conformation from several proteins simultaneously.
We show in this paper that all-α transmembrane protein secondary and super-secondary structures can be modeled with a multi-tape S-attributed grammar.
The enumeration model grows larger super-secondary structure patterns (SSPs) by combining pairs of smaller patterns, a process that approximates a potential path of protein fold evolution.
This cut site specificity has been hypothesized to arise from a unique, relaxed super-secondary structure in this area due to local hydroxyproline poor character.
Previously, numerous studies have attempted to identify the factors that determine the stability and specificity of SSE associations through analysis of super-secondary structure model systems like β-sheets and coiled-coils.
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