Several functional genomics studies have been performed in chickpea to identify the abiotic stress-responsive transcripts by approaches such as suppression subtractive hybridization (SSH), Super serial analysis of gene expression (SuperSAGE), microarray, and EST sequencing [ 7- 9].
Mitsuya et al. [ 14] and Sagor et al. [ 17] found that a number of genes in Arabidopsis showed response to exogenous application of Spm and Cucumber mosaic virus (CMV) infection based on the super serial analysis of gene expression (SuperSAGE), implying that Spm-mediated signaling pathway might play a role in CMV response.
More recently however, a super-resolution analysis of 15 platelet α-granule cargoes using 3D structured illumination microscopy (3D-SIM) failed to confirm any functional co-localisation [126].
In addition, super-resolution analysis of human centrosomes has mapped the localization of γ-tubulin towards the N-terminus of PCNT (Lawo et al., 2012; Mennella et al., 2012; Sonnen et al., 2012).
More recently, a super-resolution analysis of 15 platelet α-granule proteins using 3D structured illumination microscopy (3D-SIM) however failed to show any functional co-clustering (Kamykowski et al, 2011).
Applying Mosaic-seq to 71 constituent enhancers from 15 super-enhancers, our analysis of 51,448 sgRNA-induced transcriptomes finds that only a small number of constituents are major effectors of target gene expression.
(D G) Dual-color 3D-SIM super-resolution imaging analysis of HeLa cells (D) immunostained with anti-Trak1 antibody (green) and anti-TOM20 antibody (red); and shCTRL- or shTrak1-transfected HeLa cells (E) labeled with DsRed2-mito (DR-mito; red) and anti-TOM20 antibody (green).
Further information on single molecule particle tracking methods can be found in reviews by Shuang et al., Saxton and Jacobson, and Chenouard et al. Super-resolution imaging analysis of TIRF data can obtain spatial resolutions as small as ∼10 nm.
The super-resolution imaging analysis of Gravin null MEFs in Figure 3F argues strongly for the latter as these cells are deficient in astral microtubules but contain equal amounts of a canonical PCM protein, pericentrin ((Doxsey et al., 1994); Figure 3E,G).
These results provide important information for the wind-induced vibration analysis of super tall buildings.
