Thus, we engineered KPI variants for controlled retention in fibrin biomaterials, using either covalent binding through incorporation of a substrate for the coagulation transglutaminase Factor XIIIa or through engineering of extracellular matrix protein super-affinity domains for sequestration into fibrin.
By the use of the ultra-high specific activity [11C]Ro15-4513, the super high affinity binding sites in the rat brain hippocampus could be clearly visualized even at the extremely low concentration of 0.66 pM Ro15-4513 by in vitro autoradiography.
More interestingly, a trend for competition was observed between H-THK5117 and FDDNP at the super-high affinity binding site.
Increasing concentrations of FDDNP showed a trend for competition with H-THK5117 for a super-high affinity binding site (picomolar range) and a low affinity site (micromolar range; Additional file 3a).
He even mentioned his own complex: big ears, which explained his affinity for Super Mario, a.k.a. the Sri Lankan god statue with the huge lobes.
This research proposes a novel trimming strategy and process for identifying new product concept by trimming and integrating various systems at super-system level using affinity index and Dendrogram.
He's the Dim Mak dancefloor deviant with a natural affinity for super-charged electro beats.
Batch purification of both 6xHis-tagged proteins was achieved by His-tag affinity chromatography using Super flow Ni-NTA resin under denaturing conditions.
If Arg85 is really involved in generating the additional stability/interaction, then, owing to cooperativity, mutation of this residue would have a greater effect in the super-agonistic variants (decrease in binding affinity) compared to wild-type IL-4.
This week we're channeling Duvall's Millie Lammoreaux, a wide-eyed, chatty, ditzy loner with an affinity for rust-colored makeup, super 70s bobbed hair and a deep tan.
Maximum WCE (91.4%) was recorded with Affinity and 2,4-D + puma super, irrespective of the tillage intensity.
