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The set of pairwise phylogenetic distances between all the A proteins in M can be thought of as representing the evolutionary history of those proteins, via sums of branch lengths in an implied phylogenetic tree.
Under a free recombination model, the three recombination events would have the same probability because the sums of branch lengths of clonal genealogy between the arrival and departure points on the clonal genealogy are the same.
Both distance matrices were then analysed with FITCH, which estimates phylogenies from distance matrix data under the "additive tree model" according to which the distances are expected to equal the sums of branch lengths between the species, using the Fitch-Margoliash criterion.
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An analysis of the 6-second runtime of PhyClass on the S500R data set shows that more than half is consumed reading and writing text files, with the remainder divided between distance and sum-of-branch-length calculations, the former of which can be factored to a set-up phase instead of being done on a per-query basis.
The optimal tree (with the sum of branch length = 2.424) is shown.
The optimal tree with the sum of branch lengths = 2.73 is shown.
The optimal tree was built using Neighbor-Joining method (sum of branch length = 0.2448).
The optimal tree with the sum of branch length = 181.44778756 is shown (next to the branches) in the Fig. 2.
This method is based on principle of finding pairs of operational taxonomic units (OTUs), "neighbors" that minimizes the sum of branch lengths at each stage of clustering of OTUs.
Then the patristic distance, which is the sum of branch length on a path between a pair of taxa in the phylogenetic tree, was extracted using software PATRISTIC [23].
The sum of branch support scores for the eight nodes is +10, but the simultaneous collapse of all eight nodes in a single tree requires only two extra steps.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com