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Input: the input to ALCHEMY is the summary intensity values for the A allele and B allele channels, for all SNPs interrogated by the assay.
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Otherwise, expression summary intensities, such as those processed generated by MAS5 (Affymetrix) or dChip were processed [37].
Bead summary intensities were log2-transformed and normalized using quantile normalization [ 22, 23].
Bead summary intensities were RMA-background corrected [ 40], log2-transformed and normalized using quantile normalization [ 41].
Bead summary intensities were log2-transformed and normalized using quantile normalization [ 19].
Prior to running ALCHEMY, the actual raw data that is platform dependent is converted into these summary intensities.
Raw gene array bead summary intensities were extracted using Beadstudio version 3.2 (Illumina) and quantile normalized in R/Bioconductor.
Normalized bead summary intensities were imported in BRB-ArrayTools (developed by Dr. Richard Simon and BRB-ArrayTools Development Team) for further analysis.
Using BeadStudio software (v3.2+), summary intensities for each bead type on the array were produced, and quantile normalization between samples was performed.
This proprietary format is output directly from the scanner and stores summary intensities for each probe-type on an array in a compact manner.
The Microarray Analysis Suite (MAS Version 5.0; Affymetrix) was used to generate.cel files for each of the RNA hybridisations by scanning and computing summary intensities for each probe without the use of probe mask files.
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