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To calculate absolute mRNA expression levels, we used the RMA (Robust Multiarray Averaging53) method provided through R library Affy to obtain background adjusted, quantile normalized and probe level data summarized values for all probe sets.
The least square method and nonlinear regression analysis based on the summarized values in Table 1 were used to determine the kinetic model parameters from the experimental data provided in Table 2.
The raw data were normalized using the robust multi-array average (RMA) [19] background-adjusted, normalized and log-transformed summarized values.
The summarized values are an average taken across all exons.
Accelerometers sampled at 100Hz and summarized values for the selected variables every 5 seconds.
Twenty imputations were performed to allow for the uncertainty of imputed data and the summarized values were then used [ 19].
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Summarization of gene expression data was performed by implementing the robust multichip averaging algorithm, with subsequent baseline normalization of the log-summarized values for each probe set to that of the median log summarized value for the same probe set in the control group.
Table 7 summarizes values of the objective function for each coalition obtained from the CoMDVRP.
Table 2 summarizes values of Ki and V 0) obtained by previous workers using computer modelling and Patlak-Rutland analysis.
For instance, gene expression differences may appear as alternative transcripts and be missed in summarizing values.
This is done by summarizing values from all gene symbol BCS categories from the list.
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