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To summarize, a growing number of antisense transcripts with an established function have been identified but still many questions remain concerning their mechanisms of action.
Probes signal were summarized at a transcript level using median polish.
Intensity data were imported, log2-transformed, and quantile normalized using RMA [ 31], and expression levels were summarized on a transcript level using the mean value of all probesets mapping to a transcript.
Comparisons were made either on the exon-level to investigate alternative splicing or on the whole gene-level to summarize all transcripts of the gene.
To summarize transcript stage specificity in a single quantitative metric, we calculated a maximum transcript specificity score that is based on the Jensen-Shannon (JS) divergence.
The parameter mergeGroups determines whether to summarize at transcript level (TRUE) or probeset level (FALSE).
Our study used the Affymetrix Human Exon 1.0 ST Array, which was designed to measure every exon in the gene and then summarize them into transcript level measurements based on known transcript composition.
To summarize transcript level variation of these approximately 3000 genes, we employed factor analysis.
In this work we align reads to the transcriptome at the isoform level and we summarize transcript abundance at the gene level.
Affymetrix Human Gene Array 1.0 ST arrays were processed with the oligo Bioconductor package (Carvalho and Irizarry, 2010) to summarize probe-set transcript clusters.
A transcript summarizing the main findings was sent for review and revision to the interviewees.
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