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Arrays were normalized using Quantile normalization and probeset summarization was done using Median Polish.
Probeset summarization was done using ExonRMA16 algorithm with confidence level set at 100%.
Data collation and summarization was done in two steps, as recommended methodological procedures found in the literature [ 38, 40].
Preparation was done in R. All Affymetrix data sets except GSE4732_p1 were processed with the affy package [ 34] from raw data, and expression summarization was done with the rma algorithm [ 35] with quantile normalization.
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The background estimation and the probe summarization were done on the raw data from each chipset separately according to the RMA algorithm [34].
We note that summarization is done at the probe set level.
Summarization is done with the ExonRmaPlm function [ 35].
Background adjustment, normalization and summarization of the microarray data was done using the GCRMA method [ 34].
Descriptive analysis was done through summarization using percentages (frequency distribution), tables, graphs of the study variables and summary statistics.
Briefly, RMA summarization of probe level data was done by performing three individual treatments on all of the experimental CEL data in succession.
Pre-processing (including quality control) and expression summarization for the Illumina data sets was done with the lumi package [ 38] applying a vst transformation [ 39] and quantile normalization.
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