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Illumina's BeadStudio software (version 1.5.1.3) was used to process raw array data according to [ 31] and to output a single summarised value for each of the 48,000 genes on each array.
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When summarising values across the different countries, the central tendency and spread of the data are primarily expressed as medians and interquartile ranges (IQR), respectively.
The raw data was normalised using the robust multi-array average (RMA) [ 14] background-adjusted, normalized and log-transformed summarised values as first suggested by Li and Wong in 2001 [ 15].
The summarised intensity values obtained by illuminaio are nearly identical to those reported using GenomeStudio.
Probe sets were processed for differential expression (DE) by using the two-class significance analysis of microarrays (SAM) [ 15], based on GC Robust Multi-array Average (GC-RMA) summarised expression values [ 16].
The BeadStudio matrix contains the summarised expression values (Avg_Signal), standard error of the bead replicates (BEADSTDEV), number of beads used (Avg_NBEADS) and a detection score, which estimates the confidence limit of detection of a gene.
Hawking, with characteristic elegance, summarises its value: "Even more important than the freedom of speech is the freedom to speak".
This review summarises the value of magnifying endoscopy in the upper and lower gastrointestinal tract and focuses on gastroesophageal reflux disease and early gastric and colorectal cancer.
Precisely the importance of this concept summarises the value taken on by design at the border between formal and informal.
Then follows discussion of the general issue of Māori representation in relation to the history of Washday, and the article concludes by summarising the value of Washday to Aotearoa New Zealand.
Finally the score (0 7) was calculated by summarising the value of intensity and the category of positively stained cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com