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Determined from a Fractional Factorial Design (FFD) approach, yield, sum of peak areas, and peak numbers were rather meaningless responses.
For each fragment, we compute its weight as the sum of peak intensities of the corresponding peaks.
For the fractionation step, a solid phase extraction method was optimized by a Box-Behnken Design (BBD) approach using the sum of peak areas as response.
Monoester 1, 2 and 3 are the sum of peak areas in these regions after subtracting the peak areas for identified P forms.
R was defined as the ratio between the distance of peak values and the sum of peak widths at half height, with the second peak as reference.
Note that a particular fragment can be very common, yet have relatively small sum of peak intensities, because fragments peaks are consistently of small intensity.
Similar(34)
Although DNA concentration was controlled during the preparation of the plates, for data analysis each sample was normalized internally by dividing the height of each individual peak by total sum of peaks heights (dosage quotient).
Each integrated peak was normalised so that the total sum of peaks was set to 1000.
The result was exported into Microsoft Excel 2007, where each peak was normalized to the total sum of peaks.
S/G ratios were determined by summing the peaks ascribed to syringyl moieties (namely, m/z 154, 167, 168, 182, 194, 208, and 210) and dividing by the sum of peaks ascribed to guaiacyl-derived units (m/z 124, 137, 138, 150, 164, and 178).
The sums of peak intensities of each m/ z are shown as gf3 (DP4), gf4 (DP5), gf5 (DP6) and gf6 (DP7) in Fig. 6.
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