The parenchyma score (0 9) was the sum of the extent of dense parenchymal opacity (0 3), the extent of ground glass opacity (0 3) and the extent of cysts or bullae (0 3).
The total score was evaluated by the sum of the extent and intensity of the staining (score = extent + intensity) of seven fields in sections that were stained with the respective antibody using a 100× magnification selected from each section of the colon per rat.
Acute lung injury score corresponds to the sum of the extent (score 0, 1, 2 and 3 for an extent of 0%, <25%, 25 50% and >50%) and the severity of lung injury (score 0 for no changes, score 1, 2 and 3 more severe changes).
For the peribronchial thickening score (0 9) the sum of the extent of peribronchial thickening in the central lung (0 3) and the extent of peribronchial thickening in the peripheral lung (0 3) was multiplied by the severity of peribronchial thickening (1 1.5).
The injury score represents the sum of the extent and the severity of injury.
Pathological categorization of ERβ1 levels was determined as a sum of the extent and intensity scores.
In brief, the subscore for bronchiectasis (possible range: 0 12) was defined as the sum of the extent of bronchiectasis in the central lung (0 3) and in the peripheral lung (0 3), multiplied by the average bronchiectasis size (0 2).
Those locations were confined within either: 1) a central disk (i.e., 0 1° radius) or 2) a mid-eccentric ring (1 10°) or 3) the sum of spatial extents 1 plus 2 (0–10°).
At the beginning of each block, subjects were cued with a message on the screen (4 s duration) indicating that the target dot appeared within ether: 1) a central disk (i.e., 0 1° radius) or 2) a mid-eccentric ring (1 10°), or 3) the sum of spatial extents 1 and 2 (0–10°).
The final score was defined as the sum of both parameters (extent and intensity), and grouped as negative (score 0 and 2) and positive (score 3 6), as previously described [ 24].
