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Estimated Optical Fractionator count was calculated as the sum of cells in all five regions.
The cell counts and the size of the dissector used to estimate the density, were given by the general formulae N = ∑C/Vf, where N = estimate of numerical density, ∑C = sum of cells counted, and Vf = volume of the disector.
The results presented represent the sum of cells of all three counting fields.
An average value for each material was obtained from the sum of cells counted in ten separate areas.
To quantify invasion, CMTMR fluorescent cells in the sections above 20 μm were added and then divided by the sum of cells in all the z sections.
Circulating mDCP and pDCP were detected due to their specific staining for BDCA-1 and BDCA-2 in regions R3 and R4 and total DCP (tDCP) were the sum of cells in region R3 and R4.
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Therefore, in the present study, the total number of cells in each of the regions illustrated in Fig. 1 was quantified, and the sum of cell numbers for each region and in both directions was defined as the estimated number of cells in each region.
The sum of cell stained scores and intensity scores was calculated to quantify the expression of Itgb3.
The NS size reduction suggests that the net cell growth within the NS, which reflects the sum of cell divisions from self-renewing NSC and NPC, was impaired.
When Gorter and Grendel compared the surface covered by the lipids to the estimated sum of cell surfaces, they found a 2 1 ratio.
Hence, for example, the density of the amo gene (associated with aerobic ammonium oxidation) would be the sum of cell densities for all cell species capable of producing the amo enzyme (i.e., in the context of FBA, capable of potentially performing the amo reaction) multiplied by the number of amo gene copies per cell.
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