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Interestingly, the lowest read frequency can be predicted accurately (rho = + 0.79, P < 0.001, N = 36) from the ratio between the lowest allele amplification efficiency and the sum of amplification efficiency across all alleles constituting one genotype.
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The sum of the size of each amplification product was also compared to the TVR from normal DNA.
f Total sum of ORFs targeted However, if the amplification was clone-based and the expected PCR product was not obtained, the template DNA was sequence controlled.
b The overall number of deletions and amplifications is the sum of those found in all strains, so indels found in more than one strain were counted multiple times.
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Dept. of Amplification.
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In a Dept. of Amplification Jan.
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