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These ray sum images are without magnification and distortion.
For anatomical reference, the bioluminescence, fluorescence, and SPECT sum images are shown in color on top of the grayscale photographic image for pixel values above an adjustable threshold.
Au + Hg Mα sum images were transformed into a matrix in R, a freely available open source data analysis toolset [14], and processed using the {denoise.modwt.2d} function with the Haar wavelet selected as the analyzing wavelet.
Figure 7 presents the imaging results obtained from the different modalities in both U-SPECT-BioFluo and IVIS. Figure 7A shows respectively the bioluminescence, fluorescence, and SPECT sum images recorded with U-SPECT-BioFluo.
Because the thickness of the Au and Hg on the surface represents a small fraction of the X-ray activation depth, the resulting sum images are not of high quality and are dominated by Poisson noise.
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Pixels with high-intensity value in the sum image represent motion parameters supported by many features.
Overlay of the SPECT sum image was done by matching the mouse contours to the grayscale images of the mouse.
The sum image for Au + Hg M line X-rays is therefore a compositional image that mimics daguerreotype photograph contrast.
This algorithm forms a nonlinearly weighted sum image from both original image and its local spatial and gray level neighborhood.
For comparison to the optical images, a (2D) SPECT sum image is made by summing the voxel values along the vertical direction.
The two filter output images obtained with these two masks are summed, and the triangular object is recovered from the sum image, as shown in Figure 7f.
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