Dr Stephen P Ethier generously provided SUM cell RIPA lysates from the Karmanos Institute.
In sum, cell death in response to multiple stresses can be counteracted by 14-3-3 14-3-3 14-3-3
SUM cell lines were obtained from Dr. Stephen Ethier (Kramanos Institute, Detroit, MI, USA) and are commercially available (Asterand, Detroit, MI, USA).
The percent coverage of EC surface by pericyte cell bodies was calculated by dividing the sum cell body/process length by the EC surface dimension.
SUM cell lines were obtained from Dr Stephen Ethier (Kramanos Institute, MI, USA) and are commercially available (Asterand, Detroit, MI); the MDA.MB.231, MCF7, and MCF10A cell lines were purchased from American Type Culture Collection (Manassas, VA, USA).
Core area is calculated by ranking cells by their abundance value from greatest to least, then summing cells with the highest abundance values until the total is equal to or greater than 50% of the total predicted abundance for the entire study area.
We found that summing cell responses is strongly suboptimal and that heterogeneity in tunings is information rather than noise.
Cell growth and proliferation analyses showed that knockdown of ERLIN2 slowed the proliferation rate of SUM-44 and SUM-225 cells, but had only a minor effect on SUM-102 and MCF10A cells, which lack ERLIN2 amplification.
SUM-190 and SUM-225 cells were obtained from Dr. Stephen Ethier (Wayne State University, Detroit, MI, USA).
SUM-149 and SUM-190 cells were a gift from Dr Sofia Merajver (University of Michigan, MI, Ann Arbor, USA).
