Exact(5)
The 30 and 70% ammonium sulfate fractions were pooled and then subjected to MonoQ anion-exchange chromatography Significance of the difference (P ≤ 0.05).
Exposure to elemental carbon, organic carbon, nitrate, and sulfate fractions of fine particulate matter and risk of preterm birth in New Jersey, Ohio, and Pennsylvania (2000 2005).
Control reaction of intracellular crude extract and 40%% ammonium sulfate fractions contained 100,000 oocysts and phosphate buffer 20 mM pH 7.2 (up to 1 ml).
To separate the two activities by column chromatography, the 40 80% ammonium sulfate fractions of cancer patient serum (3 mL), containing sialidase and inhibitory activities, were loaded on Octyl-Sepharose (1.5 × 3 cm) (Fig. 4b).
To determine specificity for any other type of sialidase, the 50 80% ammonium sulfate fractions were incubated with equal amount of each recombinant sialidases with ganglioside GM3 (Fig. 3b, left) or 4MU-NeuAc (Fig. 3b, right) as substrates at an appropriate pH.
Similar(55)
Figure 8 Plot of sulfate fraction produced by shallow sulfide oxidation vs. parent water body fraction.
Detailed legend: Plot of sulfate fraction produced by shallow sulfide oxidation vs. parent water body fraction.
Almost 100%% of activity was recovered in a 0%to30%30 % ammonium sulfate fraction with 7.8-fold purification (Table 1).
Increase in sulfate fraction produced by shallow sulfide oxidation accompanied by decrease of parent water fraction points to dilution combined to sulfide oxidation by oxygen from shallow water.
Increase in sulfate fraction produced by shallow sulfide oxidation at constant parent water fraction points to near surface sulfide oxidation by atmospheric oxygen.
The ammonium sulfate fraction precipitating between 0 and 30 % saturation was washed with 50 mM sodium phosphate, 5 mM dithiothreitol (DTT), 30%% saturation of ammonium sulfate buffer, pH 7.0 on ice.
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