Sentence examples for sulfate buffer from inspiring English sources

The ammonium sulfate fraction precipitating between 0 and 30 % saturation was washed with 50 mM sodium phosphate, 5 mM dithiothreitol (DTT), 30%% saturation of ammonium sulfate buffer, pH 7.0 on ice.

Total cell lysates were prepared in a 1x sodium dodecyl sulfate buffer.

As doxorubicin leads to accumulation of iron in the mitochondria [ 46], we used ferrous sulfate buffer for CLox-positive control.

The column was washed with 100 mL of ammonium sulfate buffer [1 M (NH3 2SO4, 20 mM Tris-HCl (pH 7.9), and 5 mM MgCl2], and protein was eluted with a 200 mL linear gradient of 100 to 0% ammonium sulfate buffer.

One ml of the sample was diluted 20 times in 1.7 M Ammonium Sulfate buffer and then loaded on to the Hydrophobic interaction column column (1 ml Phenyl Sepharose Fast Flow, low substitution, from GE) at 1 ml/min.

The cells were fixed with 1% formaldehyde, harvested and lysed in sodium dodecyl sulfate buffer containing 50 mM Tris HCl (pH 8.1), 0.5% sodium dodecyl sulfate, 100 mM NaCl, 5 mM ethylenediaminetetraacetic acid and protease inhibitors.

B. thetaiotaomicron was quantified using a species-specific probe and shown to persist in fermenters 144 h after inoculation (relative abundance 0.48% and 1.42% of total SSU rRNA with standard and chondroitin sulfate buffers, respectively).

The reaction mixture consisted of 60  μL of 50 mM Tris-sulfate buffer (pH 7.6, containing 0.1 mM ZnCl2), 10  μL (0.5 mM) test compound in 1% DMSO, and 10  μL (50 U) bovine enzyme per well.

A lab-made 2× (0.575%) or 4× (1.15%) lithium dodecyl sulfate sample buffer was used, and a 2× buffer consisted of 0.575% lithium dodecyl sulfate (w/v), 20% glycerol and 125 mM Tris, pH adjusted to 6.8 [ 37].

For western blotting, cells were harvested by scraping, washed with phosphate-buffered saline and lysed using 1× sodium dodecyl sulfate sample buffer.

The precipitate was washed three times with Tris-buffered saline containing 0.5% Tween (TBS-T) and extracted with sodium dodecyl sulfate sample buffer containing dithiothreitol (DTT).