Your English writing platform
Discover LudwigSuggestions(1)
Exact(2)
Although none of these maize populations is fully resolved by markers, achievement of appropriately high marker density will fade as a major limitation, since transcript-derived markers have been shown to be reliable for generation of thousands of new markers per experiment [7], and suitable transcript profiling platforms exist for at least 14 crops as well as for all animal model systems.
NtTUBA6 is a suitable transcript that can be used as a house-keeping gene for semi-quantitative RT-PCR.
Similar(58)
Next-generation sequencing could greatly facilitate the identification of suitable transcripts leading to potentially useful promoters, and also of alternative splicing systems.
As a result, they were selected as the most suitable transcripts for the normalization of the target genes of biological interest further analyzed by RT-qPCR.
It is therefore also suitable for transcript analysis in non-annotated reference genomes.
Moreover, NGS is suitable for transcript profiling, combining the high throughput of serial analysis of gene expression (SAGE) with the functional annotation capacity of EST sequencing [ 30].
To attain this broad objective, we have designed and characterized a high density oligonucleotide microarray suitable for transcript profiling of various peanut tissues.
We compared the localization of these differentially-expressed transcripts in the ovaries of untreated asexual and sexual aphids using whole-mount in situ hybridization (WISH), a technique that is not suitable for transcript quantification.
In addition to 3 references genes suitable for transcript normalization in the whole developmental series, we recommended other combinations of internal controls that provide a more accurate normalization in studies focused on less heterogeneous sample panels.
First, EST-based expression analysis is not suitable for transcripts with low abundance (no or few representative ESTs in the database), which precludes statistical analysis.
The properties of the recombinant fused version, in which the small subunit was followed by the large subunit with a 10 amino acid linker, resembled those of the native heteromeric enzyme in kinetics, product chain-length specificity, and architecture, and this form thus provided a suitable single gene transcript for biotechnological purposes.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com