Thus far, a suitable method to label GCV with a PET isotope (i.e. C) is not available.
Additionally, the N-terminus of the Aβ peptide is rather flexible compared to that of the other regions of the peptide mentioned previously, suggesting the idea of the N-terminus being the most suitable site to label.
In particular, sulfonated versions of these dyes are membrane impermeant and may be suitable to exclusively label surface proteins [21].
Therefore, we designed two alternative EuE-based ligands suitable to be labeled with either chemo-selective oxime ligation (as a generally applicable 18F-labeling strategy for peptidic ligands) or established acylation chemistry with 18F-FPyl-TFP.
In the first strategy, the G-rich oligonucleotides were labeled with fluorophores suitable to serve as FRET partners and the probes obtained were called PSO (potassium-sensing oligonucleotide).
Being an immune-affinity-based method, QD labeling is typically suitable to detect endogenous proteins, in contrast to the earlier mentioned genetic-based labeling approaches.
Since each template can only accommodate a fixed number of labels, an important task is to work out the compositions of templates by allocating suitable labels to each template in order to fulfill the order requirements effectively.
Despite its short half-life relative to Cu, for a polypeptide with quick pharmacokinetics, the physical half-life of F-18 is more suitable for labeling polypeptide to meet the requirement of radiolabeling of polypeptide agents in PET [ 1].
Similarly, recent advances in the development of DNA biosensors using gold nanoparticles to improve DNA immobilization on electrode surfaces and as suitable labels to improve detection of hybridization events are considered.
Furthermore, we tested different lectins (UEA I, HPA, WGA) for their suitability as markers for the correct apical polarization of the GECs, and we found WGA to be the most suitable lectin, labelling the apical cell membrane reliably.
