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To test whether P tcu-1 is suitable to knock down expression of an essential gene and recapitulate loss of viability, we inserted P tcu-1 into the 5′ region of the essential gene hpt-1, which encodes the sole histidine phospho-transferase in N. crassa (Banno et al. 2007; Borkovich et al. 2004).
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For example, the creation of P. pastoris strains providing a fully humanized glycosylation pattern necessitated in the first place the development of suitable genetic strategies to knock out and introduce multiple genes [ 46].
Recombinant single-chain variable fragments (scFv) antibodies represent powerful tools for different immunotherapy purposes and are particularly suitable in intracellular immunisation to knock out specific protein functions [ 8- 11].
Another character, Trish, steals a coat from her stylish, irreverent housekeeper, hoping to knock her confidence down a peg to a level more suitable for a servant.
Marjorie agreed to knock.
"You're supposed to knock.
To knock out those possibilities.
I wanted to knock the silos down.
I want to knock him out".
This movie wants to knock you out.
But that's not to knock them.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com