Exact(1)
As detailed in the Supplemental Experimental Procedures, identifying the cases more suitable to experimental validation narrowed our candidates down to mutations on three positions in direct contact with the substrate and high KINspect score (Creixell et al., 2015) leading to the cloning, expression, and purification of these mutant kinases as well as their wild-type variants.
Similar(7)
What Ms. Parsons and her company -- led by Al Pacino (as Herod), Marisa Tomei (as Salome), Dianne Wiest and David Strathairn -- have devised is a strange, shrewdly stylized interpretation of a densely lyrical text that would seem more suitable to an experimental theater downtown than to Broadway.
In silico methods, such as molecular dynamics (MD) simulations, pharmacophore modeling or docking, proved to be suitable to complement experimental approaches.
One impressive advantage of this experimental model is that the different features of the pathology appear rapidly, all within around one week after the induction of the pathology, making suitable to perform experimental approaches to halt the scaring process.
Preliminary QM/MM calculations [HF/6-31G d,p):UFF] ensured that this cluster size leads to convergence and is suitable to reproduce experimental ADPs at low temperature.
Among the analysed two-parameters models, the Freundlich model was considered the most suitable to fit experimental data in all cases; the highest R values and the lowest values of other error functions are shown (Table 2).
Although organisms might respond to naturally occurring oxidative stress conditions [ 15] or endogenous ROS [ 3] in a quite different way these reactants are highly suitable to optimize experimental conditions and, hence, to improve the reproducibility of the size and intensity of stress sensed by cells [ 6, 7].
These results indicate that the PGM sequencer produces adequate coverage to detect highly enriched regions of methylation and suggest that the Ion Torrent-compatible MeDIP-Seq protocol is scalable simply by using semiconductor chips of varying sequencing capacity suitable to individual experimental needs.
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