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A major impediment to our understanding is obtaining suitable tissues for study.
A complete analysis would involve crossing the Smchd1 mutant allele onto a suitable mouse strain carrying many SNPs in imprinted genes for determining allelic expression and analysis of suitable tissues where their expression is imprinted.
The liver is one of the most suitable tissues in that it participates more than other organs in a range of physiological processes and contains cell types endowed with distinct roles and functions.
Splice variants were enriched for EST originating from different cDNA libraries and, for a given gene, suitable tissues were chosen according to the origin of ESTs for the minor splice variant or the expression profile found in the Stanford SOURCE data base [ 79].
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However, there are several requirements that have to be fulfilled in order to develop a suitable tissue engineered implant.
The growth of suitable tissue to replace natural blood vessels requires a degradable scaffold material that is processable into porous structures with appropriate mechanical and cell growth properties.
Conversely, sparse gels (≤1 mg/ml), which are extensively used in 3D cultures, failed to reproduce the hindered diffusion of tissues, thereby supporting that dense (but not sparse) ECM gels are suitable tissue surrogates in terms of macromolecular transport.
We aim to tissue engineer heart valves using a hydrogel scaffold based on poly vinyl alcohol) (PVA), and the design parameters for a suitable tissue engineering scaffold are quite stringent.
It was shown to be a suitable tissue for the diagnosis of bacteremia in guinea-pigs experimentally infected by the intracellular bacterium Coxiella burnetii [4].
In fact, Mirón et al. [24] showed that whole blood is a suitable tissue to track seasonal dietary shifts in bats.
Understanding the mechanisms leading to such organization is an important step in learning how it happens in the formation of natural tissues and for developing strategies to engineer suitable tissue equivalents.
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