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Therefore, it is necessary to search for appropriate contrast agents and to develop suitable staining protocols, which finally allow segmenting the vessel tree.
The cells were incubated with substrate solution at room temperature until suitable staining developed.
Areas with suitable staining were prepared for electron microscopy and viewed on a Phillips CM10 transmission electron microscope.
Next, alkaline phosphatase streptavidin (Vector Laboratories Inc., Burlingame, USA, Cat. No. SA-5100) diluted 1/900, was applied for 50 min. After washing once in TBS, twice in TBST, and twice in TBS, colour reaction was performed using BCIP/NBT Kit (Vector Laboratories Inc., Cat. No. SK-5400) until suitable staining developed 15-255 min).
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Such dyes might not be suitable for staining the fine details of all parts of the plasma membrane of adherent cells, both at the free surface and the cell bottom.
Most of the results presented here are based on DAPI-stained nuclei (i.e., RGS), despite its preferential binding to AT-rich regions of DNA, because only dried material was available from most populations, which is not suitable for staining with the intercalating fluorochrome propidium iodide.
The importance of ease of synthesis in order to overcome the difficulties associated with their trial and error optimisation in cellulo was inadvertently exemplified through the disparate behaviour of sensors 6– 8, all of which were originally anticipated to be suitable for staining the plasma membrane in biological systems.
Even baby wipes or personal wipes will remove many a stain if you can't find suitable portable stain removal items.
They are suitable to stain selectively lysosomes of tumor cells and monitor its pH changes sensitively via optical molecular imaging.
For selection of suitable host stains, pEXP-hCRs were transformed into E. coli strains BL21, C41, C43, C41-pLysS and C43-pLysS, respectively; while for pBAD-hCRs, the E. coli strains Top10 was recommended by manufacturer's instruction.
The cells were then stained with an anti-IFNγ mAb or an isotype control suitable for intracellular staining experiments (mIgG1, MOPC-21; Biolegend) for 30 min at 4°C.
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