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This approach is limited by availability of a suitable screen and the requirement that the desired trait be expressed in the host cell.
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As illustrated by the ATHase and metathesase, each ArM behaves differently, and exploratory screens should be performed to identify the most suitable screening strategy.
Also for the efficient use of the plates suitable screens or colour-filters are required to equalise the action of the various colours.
Although the enzymes' S/H ratio can be increased by protein engineering, such approaches require a suitable screening assay.
Although an enzyme's substrate specificity can be altered and broadened by protein engineering and directed evolution, this requires a suitable screening assay.
However, relatively few studies on directed evolution or saturation mutagenesis of glycosyltransferases have been reported in part due to a lack of suitable screening methods.
It also investigated the most suitable screening concentrations of FOX, ceftriaxone (CRO) and ceftazidime (CAZ) for the detection of AmpC β-lactamases.
Having established that type IV collagen was a suitable reporter for our shRNA screening, we carefully sought a suitable screening strategy, since a previously reported gene screening using a Polycomb-target gene as the reporter failed to enrich most Polycomb-group genes17.
Moreover, drought stress breeding is challenging due to the lack of suitable screening methods.
Identification of suitable screening tests for substances with production volumes below the threshold value (Fig. 1, step 1).
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