The constructs are designed to the base pair and are not dependent on suitable restriction sites.
Therefore, a subject for future work will be a control method to set a suitable restriction angle based on the step height and road-surface shape.
For the two-phase model positivity of volume fractions in general is not ensured, and a suitable restriction on the CFL number might be needed.
Finally, a rational cloning strategy must be designed prior to each cloning event by identifying a compatible vector and suitable restriction enzymes.
Even though the first problem is nonconvex, we identify a suitable restriction of its feasible set that makes it convex while maintaining the same optimal value.
Based on an in silico restriction analysis of database sequence entries, suitable restriction enzymes for a T-RFLP approach were selected.
With some suitable restriction on f, we can weaken the condition about smoothness property of results obtained in [1], and just require the differentiability on the domain of the operator.
The full-length TuMV cDNAs released from the p35S-TuMV series were constructed downstream of the CaMV 35S promoter (35S) by insertions in between suitable restriction enzyme sites of pBD003.
The 5′ flank was ligated to the front (XhoI/EcoRI) and 3′ flank behind (NotI/SacII) the pyrG gene, respectively, using suitable restriction enzymes (Thermo Scientific) and T4 DNA ligase (NEB).
The full length of TuMV cDNA was constructed in pBD003, downstream from the CaMV 35S promoter, with suitable restriction enzyme digestion and ligation to generate an in vivo pBD-TuMV infectious clone.
These approaches do not rely on the presence of suitable restriction sites and can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule.
