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The authors used LC MS/MS to identify 129 serum proteins which were considered suitable for relative quantification.
Gene expression was assessed by Real Time-PCR using an ABI Prism® 7000 Sequence Detection System and TaqMan® technology suitable for relative gene expression quantification as described in Materials and Methods S1.
Expression was assessed by real-time PCR using the LightCycler® 480 Real-Time PCR System (Roche Diagnostics, Barcelona, Spain) and TaqMan® technology suitable for relative gene expression quantification.
Gene expression was assessed by real-time PCR using the LightCycler® 480 Real-Time PCR System (Roche Diagnostics, Barcelona, Spain) and TaqMan technology suitable for relative gene expression quantification following manufacturer's protocol.
In addition, the slopes of stimulated and unstimulated samples, an indicator of PCR amplification efficiency, were slightly different suggesting that conventional ∆∆Ct or standard curve methods are not suitable for relative quantification of SH2 binding.
On the other hand, this approach is only suitable for relative quantification and each sample usually requires manual work (e.g., for cell detachment, transfer, washing steps) implicating a reduced number of processable samples compared to the microplate format.
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These sensors are suitable for detecting relative DNA damage rates in <5 min of the enzyme reactions.
This modular concept of functional units, allows optimized management of computation (such as identification of parallelizable functions or functions that are suitable for acceleration) relative to the hardware architecture (CPU, GPU, FPGA, etc).
Similarly, both the inverse Gaussian (IG) and Weibull (WB) are likely suitable for modelling relative frequency of mahogany dispersal events at other sites.
We therefore believe that this kind of comparison is suitable for a relative assessment within our two species.
Independent empirical methods for measuring relative ASE such as Pyrosequencing and qPCR can be used to validate RNA-seq data for individual genes, but they are not suitable for quantifying relative ASE on a genomic scale.
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