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These results indicate that Cal-520 can elicit calcium signals with higher SNRs and better temporal resolution than OGB-1 and is therefore suitable for calcium imaging of Purkinje cells in vivo.
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This same design is also suitable for quantifying dendritic calcium dynamics and tracking arbor-wide movement of any subcellular substrate of interest.
Although aequorin can be used to determine total calcium concentrations in fungi (Nelson et al., 2004), so far as we are aware, there are no versions capable of distinguishing vacuolar from cytosolic calcium nor are there calcium indicator chemicals suitable for use in filamentous fungi which can indicate the intracellular distribution of calcium.
This article describes the design, synthesis and characterization of an optical sensor suitable for practical measurement of ionized calcium in serum and whole blood samples.
This architecture makes the SMA exquisitely suitable for investigating smooth muscle cell calcium regulation in small vessels (< 70 μm) using intact arteries, as opposed to isolated cells, which are devoid of their natural milieu.
The fluorescently tagged L cells are suitable for patch clamping, single-cell dynamic calcium imaging, and cell sorting, providing a range of new and powerful techniques to interrogate the function of this enteroendocrine cell type.
As for calcium, the two most common forms in supplements are calcium citrate and calcium carbonate.
What about osteoporosis and our need for calcium?
C.M. provided FURA-2 for calcium quantification.
d Alizarin red for calcium deposition.
Less valuable for calcium is spinach, because the calcium in spinach is poorly absorbed.
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