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Instead, adults were used so that larger blood samples suitable for assay would be obtained.
Based on ranking from the in silico design criteria [ 15], 404 SNPs from 757 (53%) were suggested as most suitable for assay development, from which 384 were chosen.
However, 23% of SNPs were considered suitable for assay design with ADT scores ≥ 0.6 and confidence scores of 1 or 2, since false positive rates for confidence score 2 are reasonably low (see above).
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Therefore, this reporter construct is suitable for assaying differentiation of hESC into particular lineages in a quantitative fashion utilizing a relatively small number of cells.
It is suitable for assays in near physiological solution conditions and shows high discrimination against ADP.
A further construct suitable for assays was obtained by removal of the DUSP-UBL domain from the ΔUBL2 fusion construct.
The overall yield for full-length USP11 (FL-USP11) was poor, but pure and stable protein suitable for assays was obtained.
In their recent review on disorders of mtDNA synthesis, Freisinger et al. state that fibroblasts are not suitable for assays of depletion [ 41].
The Human Biotrak MMP-13 ELIZA kit (GE Healthcare) was used because these kits were previously shown to be suitable for assaying rabbit MMPs [ 28, 29].
In our experience, mild lysis buffers are not suitable for assaying nuclear proteins, since cell nuclei remain intact and are then sedimented by high-speed centrifugation.
Further experiences in using both buffers for goat complement showed that both are suitable for assays, but whereas DGVB++ is a good buffer for long-term storage of RBC, DGHB++ needs to be removed and changed for another buffer to store the EA cells.
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