Another popular method for studying protein interactions, isothermal titration calorimetry (ITC), lacks the sensitivity for the measurement of sub-nanomolar KD's and leads to stoichiometric binding at cell concentrations with detectable enthalpies (high " c value" conditions), unless a suitable low-affinity competitor is available for displacement experiments.
Binding was performed in presence of heparin as competitor and suitable protein concentrations were determined in pilot experiments.
The aggressive displacement hypothesis predicts that males with larger body size may rely on positive phonotaxis to displace competitors for suitable calling sites [29].
The American alligator can perform specific vocalizations to declare territory, signal distress, threaten competitors, and locate suitable mates.
Suitable corpses are widely scattered and competitors might already be closing in.
Intimidating guidelines demand competitors "be dressed in suitable tennis attire that is almost entirely white" as soon as they "enter the court surround".
Our results suggest that litter removal by the rodents gave E. chinensis suitable space that was free of competitors; this allowed expansion of the E. chinensis patches.
The rules, much feared, always enforced, say "competitors must be dressed in suitable tennis attire that is almost entirely white".
They must cope with rising wages for skilled workers, a shortage of suitable staff and pricing pressure from local competitors.
Nevertheless, using the distances to the nearest competitor or slavemaking colony is a suitable proxy of competition or parasitism risk.
