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A synthetic gene for bovine β-lactoglobulin-variant-A (β-LgA), designed to incorporate a number of restriction sites to form suitable "cassettes" for future site-directed mutagenesis experiments, was constructed as three separate fragments, F1, F2 and F3.
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An A-P X-ray is taken from the hips to the ankles with the patient standing and the patellae looking forward using a suitable cassette to gradually filter the X-ray beam in order to visualize both hips and ankles properly.
Choose a suitable cassette from the ones shown in Table 1 for the desired type of labeling (or design your own FRT-galK-FRT containing cassette).
The attR1-CmR- ccdB- attR2 cassette was generated by BP recombination between pDONR221 (Invitrogen) and a suitable cassette containing attB1 and attB2, using the Gateway ® BP Clonase™ IIenzyme mix (11789020, Invitrogen) according to the manufacturer's instructions.
To construct a suitable replacement cassette a 3.5 kb hygromycin resistance cassette was excised from pSK346 using the SfiI restriction enzyme.
We first optimized a suitable expression cassette to achieve a robust expression of gE2 by eukaryotic cells before attempting to express BVDV gE2 in a BoHV-4 based vector.
We also provide four vectors, carrying different dominant selectable marker cassettes, suitable for yeast recombination-based construction of vectors for use in Z. tritici.
To generate a STOP cassette suitable for AAV vectors, we reduced the size of the STOP cassette in the Z/EG construct [22](a gift from C. Lobe) from 4959 bp to 1788 bp.
To allow creation of inducible L4 cells from LoxP parent Clone A1, a promoterless L4 cassette suitable for Cre-mediated insertion was designed that included the L4-100K, -22K and -33K ORFs.
We switched to the pTRA vector series (derivatives of pPAM [ 21]) because they have a smaller backbone than pBin vectors (<3 kb) and are therefore more suitable for tandem expression cassettes.
For now the model contains only a suitable schematization of the Cassette Body and its objective is to analyze the effect produced by the main loads (electromagnetic loads, coolant pressure, thermal neutron and convective loads) on itself: an available estimate of loads is that one derived from ITER: for a proper translation some assumptions have been made and they are described in the paper.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com