Sentence examples for suitability of the reference from inspiring English sources
The suitability of the reference gene was then assessed by performing Kruskal-Wallis One-way ANOVA with Dunn's Multiple testing (P < 0.05) for each of the above comparisons.
For determining endogenous control of constitutive expression, the suitability of the reference genes, i.e. GAPDH (glyceraldehydes-3-phosphate dehydrogenase), 18S, and beta-actin, were evaluated with the appropriate software, i.e. Bestkeeper [ 55] and Normfinder [ 56].
This will require modifications to the scoring system which are likely to include; more stringent requirements for the numbers of biological replicates used in custom arrays based experiments, due to the larger noise variation, the use of different visualisation techniques to assess quality and an additional criterion to address the suitability of the reference sample.
The validation step was a vital exercise, not only to test the effectiveness of the SNP panel and the suitability of the reference population data, but also to demonstrate the application of the assay by a UK public analyst on case-type samples.
To illustrate the suitability of the reference genes revealed in the present study, two putative cotton homologues to AG and SEP3 (GhMand3 and GhSEP-like1) had their expression profile evaluated in different plant organs, during flower development and in floral organs at flower buds of 6 mm.
It is good practice to rigorously validate the suitability of these reference genes under the specific experimental condition to which they are applied [ 5- 7].
Beyond their robustness in T. versatilis, the suitability of these reference genes for RT-qPCR analysis within the filamentous fungal kingdom was assessed, by collecting RNA-seq based transcriptomic data from 18 phylogenetically distant fungal species.
In our study, the suitability of these reference genes was confirmed, since no significant differences between the analyzed groups were observed after analyzing the Cq mean for all four genes together and by bestkeeper [ 38].
Thus, by evaluating the suitability of a reference material, the following criteria should be taken into consideration [ 1– 5]: Homogeneity of the material.
The expression stability and suitability of the eight candidate reference genes (GAPDH, aTub, Tbp, Stx16, Xbp1, MMP, CAPON and Actin) were validated employing geNorm and NormFinder based on Spanier et al. [ 29].
The aim of this study was to assess the test-retest variability of [C]PIB studies in patients with Alzheimer's disease (AD) and healthy controls using several tracer kinetic models and to assess the suitability of the cerebellum as reference tissue.
