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In this report, we systematically evaluated the technical feasibility of using isolated CTCs for these common assay formats, with particular emphasis on the suitability of cells captured on the CellSearch® platform for these applications, since this is an FDA approved platform and now widely available in diagnostic labs.
During the initial time period of cell integration into complex culture devices and their adaptation to the cultivation conditions (often requiring days, if not weeks, to stabilize), there is an opportunity to assess the recovery and suitability of cells for particular applications.
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Our comparisons of expression profiles between breast cancer cell line subtypes and breast tumor subtypes provided valuable information relevant to the suitability of cell lines in modeling known breast tumor heterogeneity.
These results demonstrate the suitability of cell-free systems for quantifying the impact of heavy water on gene expression and establish a platform to further assess the potential therapeutic use of heavy water as antiproliferative agent.
Ultimately both the panel of (human) cell lines and viruses need to be expanded, in order to be able to predict upfront the suitability of cell lines for the production of specific viral vaccines, based on scientific understanding, thereby reducing timelines and costs in vaccine production processes.
To test the suitability of cell-based label-free techniques for this application, a panel of CNTs with different diameters and surface functionalizations was assessed by impedance-based technique (xCELLigence RTCA) and automated microscopy (Cell-IQ) compared to formazan bioreduction (MTS assay).
So far, few studies have utilized paired bronchial and nasal cells to address the suitability of nasal cells as a surrogate of bronchial cells given the impact of the genetic factors on cellular responses.
Thus these comparisons collectively confirm the suitability of CH1 cells as a model for studying mechanisms regulating BCR-induced cell cycle arrest and subsequent apoptosis in immature, transitional stage, B-lymphocytes.
The expansion suitability of chondroprogenitor cells isolated from the superficial zone of articular cartilages was evaluated in both conventional monolayer and macroporous microcarrier in spinner flask cultures.
Such comparison is essential to establish the suitability of these cells for hepatocyte applications in vitro.
The results obtained in this study confirm the suitability of CSM14.1 cells as an in vitro model for the study of neuronal and dopaminergic differentiation in rats.
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