First, some studies have suggested stabilizing natural selection on human height by way of increased health problems in very short and very tall individuals [21], [22].
Although our results suggest stabilizing selection on the F-ORF region's amino acid composition and secondary structure and support the protein-coding hypothesis, further protein-based analyses will be necessary to characterize the biological significance of the mytilid ORFs, and to verify if they are functionally equivalent.
In addition, negative correlations among codon positions inside internal coding regions suggest stabilizing selection for a GC-content level that was similar in both species.
These estimates were compared with the analogous trait-wise QST-estimates measuring genetic differentiation in phenotypic traits, where QST >FST suggest differentiation due to divergent selection and QST suggest stabilizing selection [ 25].
Notwithstanding this general trend, there were clearly instances when comparing the same two populations showed that pairwise PST was either considerably less than FST (suggesting stabilizing selection) or considerably higher (suggesting divergent selection).
Several trials provided significant evidence for negative quadratic terms (SB 2006, SB 2007 and KS 2007; P ≤ 0.10; Table 2; Fig. 3), suggesting stabilizing selection against developmental extremes at stocking.
The attraction between these positively and negatively charged regions and the hydrophobic patches around residues 60, 130, and 180 therefore suggest stabilizing interactions and consequently stronger tertiary structure propensity between residues 60 and 180, compared to other regions of OPN.
In contrast, most BSCTV isolates characterized to date, including those from various crops from Old and New World locations, have been less variable, perhaps suggesting stabilizing selection within this species [ 11, 39, 41].
This suggests that stabilizing repeat modules based on consensus information might be added to a repeat protein domain, allowing it to be extended without altering its overall topology.
Bands appearing due to phosphorus and carbon bond stretching shifted to 1384 cm−1 and became more intense which suggests the stabilizing interaction of phosphine group with gold nanomaterials.
