Despite citrate plasma being the suggested sample of choice for estimating circulating MMP9 (Makowski and Ramsby, 2003), serum sampling may still be useful providing that methods of collection and processing are standardised.
Considering this, we will need 112 participants in each arm, which is feasible to obtain from the suggested sample of 446 participants in order to detect a difference of 10 minutes with 80% power and a 0.05 significance level.
Merriam [ 6] offers precise direction for the researcher employing a case study design in terms of using a theoretical framework to define the problem, and suggests sampling of the case (and within the case) from typical or unique examples or a range of examples to achieve maximum variation.
This resulted to a suggested sample size of 90 patients based on the results of previous study [30].
This resulted to a suggested sample size of 90 patients based on the results of previous study [ 30].
It has been suggested that sampling of the penile shaft, the coronal sulcus/glans penis, and the scrotum is necessary for optimal overall genital HPV detection [ 25].
A recent simulation-based study suggested that sampling of a moderate number (up to ten) of loci could correctly delimit recently diverged biological species with a coalescent theory-based approach, even without relying on their reciprocal monophyly [ 53].
Our study suggests that sampling of benthic dinoflagellates should be conducted at different water depths taking into account the presence of the macroalgal substrate as well.
These findings suggest that sampling of soil fungal communities is more exhaustive, if we combine repeated PCR products, and PCR products generated at various annealing temperatures.
The level and duration of excretion of ASFV in the faeces, combined with the stability of the DNA, suggest that sampling of faeces could be the basis for a non-invasive sampling strategy to monitor ASFV in wild boar.
