Sentence examples for suggested concentrations from inspiring English sources
All antibodies were from Santa Cruz Biotechnology Santaa Cruz, CA) or Geneka- Active Motif (Carlsbad, CA, USA) and used at the producer's suggested concentrations.
Detection was performed with CHOP (Santa Cruz Biotech, Santa Cruz, CA, USA), KDEL (Enzo Life Sciences, Plymouth Meeting, PA, USA), XBP1 (Santa Cruz Biotech) and α-tubulin (Sigma-Aldrich, St Louis, MO, USA) antibodies at manufacturers suggested concentrations.
As for the Xist/Sry assay, all RT mixture components were used at the suggested concentrations, but volumes were halved so that RT was performed in a final volume of 10 μl.
The concentrations of inhibitors used in this assay are at least five times more than the suggested concentrations that would normally cause more than 50% inhibition of activity per mg of protein [ 43].
As previously described [ 17- 19], all RT reagents were used at the suggested concentrations except for the absence of DTT, but volumes were halved so that each assay was performed in just 10 μl, which increased to 10.5 μl after RNase H digestion.
MSP was performed in a volume of 25 μl containing 5 pmol of a primer set, 1.0 μl of bisulfite-converted DNA solution in M-Elution Buffer, and contents of ExTaq HS DNA Polymerase kit (TaKaRa) at the suggested concentrations (including 0.625 units of ExTaq HS DNA Polymerase per 25 μl of reaction mixture, 1× ExTaq Buffer [Mg2+ plus] and 250 μM of each dNTP).
Blocking peptide for the IP3R1 antibody was used at the manufacturer's suggested concentration.
According to previously suggested concentration [ 41], sodium hypochlorite experiments were conducted with a 1 50 dilution of the 6 % household bleach (Chlorox, The Clorox Company, USA), the "hypochlorite working solution".
The monkey studies suggest concentrations of 8,000 to 10,000 spores will kill half of those exposed.
Mr. Zitko's scribbles change density, direction and rhythm in ways that suggest concentration and skill, and that give each effort a different spirit and energy.
Looking across the Lone Star state as a whole suggests concentration, with six firms accounting for 91% of the branded hotels in the sample.Yet there is little geographic clumping.
