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Compared to the previously reported conjugation frequency of 9.0·10−2 transconjugants per donor in the plasmid pOLA52 [25], the observed transfer frequencies of this study suggest that the conjugation apparatus of pX1.0 functions similar to that of pOLA52.
We suggest that the conjugation of reactive electrophiles which are formed in an uncontrolled manner are deleterious to endogenous low-level signalling, and the GSH systems 'insulate' against high levels of electrophile production which may cause cellular damage.
Although the annotation process suggests that each of the plasmid classes seems to possess a different set of conjugation genes, the fact that all 11 plasmids are related, sharing large blocks of DNA sequence tends to suggest that the conjugation systems themselves will also be functionally closely related.
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This result suggested that the conjugation between monoclonal antibody and PQDs is successful.
As shown in Fig. 2a, the photoacoustic signal of MoS2 ICG was nearly 16-fold higher than that of MoS2 under 800-nm laser excitation at the same concentration of MoS2, suggesting that the conjugation of ICG significantly enhanced the photoacoustic sensitivity of MoS2 nanosheets.
The result suggested that the conjugation activity of scFvs was similar to that of parent MAb.
Previous evidence has suggested that the conjugation of the HA-CD44 receptor could activate the MAPK signaling pathway [ 18].
Furthermore, maintaining almost the same structure as HSA suggesting that the conjugation affected neither the molecular weight nor the global charge of HSA as a protein carrier.
Based on this the discoverers of pupylation suggested that the conjugation process might involve a different biochemistry, but did not specify what this reaction might be [ 1].
The in vitro susceptibility is clearly conjugation-dependent and we have preliminary data suggesting that the conjugation incompetent mutant of ISG15 does not protect cells from infection (data not shown).
This suggests that the conjugation of ubiquitin to rproteins, in addition to regulating the nuclear levels of rproteins through proteasome degradation, may also have direct or indirect effects on the ribosomal-biogenesis pathway.
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