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The urinary recovery of each sugar probe was expressed as a percentage of the orally administered dose.
To investigate the effects of LPKM on intestinal permeability, we performed the assay of TER, dextran permeability in vitro, sugar probe permeability in vivo, and Ussing chamber ex vivo.
22 Once every week, all mice were housed in metabolic cages after a 4 h fast of food and water and immediately after a gavage of 0.2 ml of a sugar probe containing 100 mg of sucrose, 12 mg of lactulose, 8 mg of mannitol and 6 mg of sucralose.
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The intestinal permeability and colonic damage in vivo were investigated using a sugar probes.
Before the ingestion of the sugar probes, the volunteers voided their urinary bladders.
Increased urinary lactulose recovery was observed both after intragastric and intravenous administration of the sugar probes.
Fluid loading has been reported to modify the excretion of sugar probes in rats [ 3].
It remains to be determined whether sugar probes are handled differently in response to fluids in patients with organ dysfunctions.
Altered excretion of sugar probes by fluid loading would invalidate the measurement of intestinal permeability in patients requiring rapid intravenous infusions of fluids.
We studied the effect of a postmucosal factor, fluid loading, on the urinary excretion of enterally administered sugar probes in healthy volunteers.
These data suggest that increased colonic permeability may be a feature for a subset of PD patients, as already reported when sugar probes were used [ 15].
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