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Two elderly sages turn up, as well; serenely dispensing dour philosophy while enjoying the devotion of young acolytes, they seem to reap the harvest of sufficiently ordered lives.
This points to the main weakness of the book, which is that its impressive erudition is not sufficiently ordered, filtered and edited to make it serve the central argument.
For instance, the crystallisation of the bacterial Zn2+-transporter Yiip was tested in the presence of several heavy metal cations and sufficiently ordered crystals were obtained only in the presence of 5 mM Zn2+ [28].
EM images of bacterial cytoplasm do not show the presence of the Z-ring, possibly because the cytoplasm is too dense to offer good contrast, or because protofilaments inside the Z-ring are not sufficiently ordered to appear distinguishable [20], [21].
Residues 139 141 of the acceptor stem loop region, which help form the active site pocket, were sufficiently ordered to model.
All crystal structures to date have been in the presence of bound tryptophan, since the apo-form does not readily form crystals sufficiently ordered for X-ray analysis.
Similar(53)
Hence, we consider charge repulsion to play the role of a "gate keeper" for those conformations of partially denatured monomers that can form energetically sufficiently favorable, ordered precursors for amyloid formation.
However, only the Alu domain and SRP54 were sufficiently well ordered to allow direct modelling into the density maps, and thus form the focus of our interpretations in the sections below.
Unfortunately, as stated in the text, uL10/uL11 are not sufficiently well ordered to confidently interpret the molecular details of the interaction surface between the ribosomal stalk and the Alu domain.
Whereas initial electron crystallographic studies focused mostly on naturally occurring 2D crystals, such as purple membranes composed of crystalline bR arrays, work on plant light-harvesting complex II showed that in vitro reconstituted 2D crystals can also be sufficiently well ordered to produce atomic models (15).
Moreover, although about a third of all macromolecules form some kind of crystal (Rupp, 2007), only a small fraction of these crystals are large enough and sufficiently well ordered to allow the macromolecular structure to be determined from a single crystal.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com