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The Rho1 protein could not be identified by this analysis, indicating that the sensitivity of the method does not allow the detection of endogenous levels of activated Rho that bind Dia, or that the extraction conditions used in the pull down were not sufficiently harsh to effectively remove membrane embedded proteins like Rho1.
Similar(59)
States passed laws making life without parole an option for certain aggravated murders, meaning there was a sufficiently harsh alternative to the death penalty.
The sun pure gold on the MedIferranean.
He appeals to the President, arguing that any interrogation permitted by the law won't be sufficiently harsh.
Alec B. Francis is nicely sympathetic as Mr. Brownlow and Lionel Belmore is sufficiently harsh as Mr. Bumble.
Philadelphia Mayor Michael Nutter has jumped into the fray, saying that he believes that a fine is not sufficiently harsh for the offense, adding that if a city official had uttered the epithet he would have been subject to suspension or firing.
Harsh to blame him for everything".
Harsh to criticise Tyson Fury though.
May need to change dissolution testing conditions (harsh to mild).
Workers who fall sufficiently sick to satisfy an increasingly harsh bureaucracy that it simply isn't feasible to class them as jobseekers are facing a benefit cut of around 30%, the sort of retrenchment more often associated with Greece.
Shortly thereafter the Rectification Campaign took, for a time, the form of a harsh purge of elements not sufficiently loyal to Mao.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com