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Cell lines were grown to confluence prior to harvest for fluorescence-activated cell sorter (FACS) analysis.
Cells were grown to confluency and harvested by trypsination.
For the present study, cells were grown to near confluency, harvested and counted.
Briefly, P. aeruginosa cells were grown to OD600nm = 3, harvested, and washed in saline.
Cells were grown to ~70% confluence before harvesting nucleic acids.
Cancer cells were grown to 80% confluence and harvested by trypsinization.
Cells were then grown to mid-log phase, OD600 ∼0.6, prior to harvest.
Cultures were grown to mid-exponential phase and harvested.
The cells were grown to 90% confluency before being harvested.
HEK293 and HEK293-CD9 cells were grown to 70 80% confluence and harvested by trypsinization.
For all assays, cells were grown to 70-80% confluence and harvested following trypsinization.
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