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However, the simulated ShakeMap appears not to have been sufficiently amplified in western Taiwan, where a thick sedimentary plain is deposited.
Since it was found that two genes PnpA like and AtlA like genes were not sufficiently amplified in PCR assay using genomic DNA from S. mutans as template, these two genes were not used in RT-PCR studies.
Some samples may have been of inadequate quality for all spacers to be sufficiently amplified.
For some DNA samples that could not be sufficiently amplified, the WGA5 Kit (Sigma) was employed.
The remaining gaps most probably contained cores of GC islands, which were not sufficiently amplified in the emulsion PCR.
The Ct value is representative of the number of qPCR cycles required for the dye-DNA conjugate to be sufficiently amplified for the fluorescence to cross the detection threshold.
Similar(53)
A pure cooperativity model (h = 0, no receptor polarization) could not sufficiently amplify the external gradient (Fig 7A).
However, to evaluate this concept of IL-7 consumption, other animal models should be developed that both detect IL-7 in peripheral lymphoid tissue, and can sufficiently amplify the signal from secreted IL-7 to permit visualization.
Biased representation could also occur by a failure to sufficiently amplify specific mRNA transcripts that exist below a detection threshold, such as very low abundance mRNA or mRNA with low hybridisation signals on the array due to a number of factors independent of amplification.
For real-time multiplex RT-PCR, if one template is much more abundant than the other templates in the same reaction tube, the reaction components will be depleted before the lower-abundance targets have amplified sufficiently to be detected [23].
Following the procedure described in Shiller et al. [36], the spectrally altered acoustic signal was amplified sufficiently in order to limit subjects' perception of their unaltered air/bone conducted speech signal (masking noise was not added due to its potential impact on the perceived noise spectrum of the fricatives).
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